Anapa has developed MeltPlex®, a Taqman-type detection method, which combines fluorometric detection with meltcurve detection, increasing analyte detection by 4-5 fold.
MeltPlex® utilizes a system of labelled Taqman-type probes allowing each to be read out by subsequent melting curve analysis. 5 or more probes can be analyzed per fluorophore channel. By utilizing melting curve readout of modified probes – one for each target - rather than the only amplicons, the system adds an extra level of specificity to melting curve analysis. Reaction and melting analysis is performed without the need to re-open PCR reaction tubes.
Using a pre-determined set of probe design rules, 16 existing Taqman-probes designed to detect a range of hemorrhagic virus strains were converted to MeltPlex® probes and combined into a single, multiplex, probe-based assay. Using 4 of the 5 channels of the Bio-Rad CFX96 instrument, we have shown proof-of-concept that the MeltPlex® system can reliably detect 16 individual viral strain sequences.
Anapa develops and validates nucleic acid technologies that improve third-party PCR-based assays. Anapa’s strategy is to license their technologies to key molecular diagnostic providers in large markets. Anapa has developed MeltPlex®, a patented Taqman-type detection method, which combines fluorometric detection with meltcurve detection, increasing analyte detection by 4-5 fold.